Animal-Model
ESTABLISHMENT OF OSTEOPOROTIC ANIMAL MODEL AND EVALUATION OF SUPER ACTIVE CALCIUM SUPPLEMENTATION
Yuseong Jang, Hyeon-Gi Paik, Jihye Choi, Ja-Jun Ku, Jungkee Kwon
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Jeonbuk National University
Eco-Biotech Co. Ltd
* Corresponding author: Jungkee Kwon (jkwon@jbnu.ac.kr)
Osteoporosis is a critical health issue prevalent among postmenopausal women. Therefore, finding safe and effective preventive alternatives is crucial. This study assessed the efficacy of super active calcium (SAC) supplementation on osteoporosis using ovariectomized (OVX) rat models.
Six-week-old rats underwent ovariectomy to induce osteoporosis, followed by a 1-week recovery period. The animals were then orally administered low, medium, or high concentrations of ionized calcium for eight weeks.
The results demonstrated that OVX significantly increased body weight, reduced uterus weight, elevated bone resorption markers (CTX1, NTX1), and negatively altered bone metabolism parameters (BMD, BV/TV, Tb.Th,Tb.Sp, Tb.N, BS/BV, SMI, Tb.Pf, SD(Tb.Th), and SD(Tb.Sp)). However, ionized calcium treatment notably mitigated these effects, improving bone density and structural parameters in a dose-dependent manner.
Serum markers of bone formation (Gla-OC) increased, and bone resorption markers were reduced compared to the OVX group. Furthermore, improvements in lipid metabolism markers (TC, TG, HDL, LDL) and hepatic enzyme levels (ALT, AST), along with normalization of serum calcium levels, were observed. In conclusion, ionized calcium supplementation significantly ameliorated osteoporosis symptoms and associated metabolic disorders in OVX rats, suggesting its potential as a safe preventive measure against osteoporosis in postmenopausal conditions.
